Insertion junction: LMJ.RY0402.086824_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.086824
Chromosome:chromosome 7
Location:2658716
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g330650 (1 of 10) PF02536 - mTERF (mTERF) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTTGCGCATGGCTTCCCCGCTCGATGGA
Internal bar code:ATGGCCCCACGGGCAGCGCCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:395
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACATAGGGTGTGGAAGCACC
Suggested primer 2:GGAGTGCCCAAAACTGGTTA