| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086824 |
| Chromosome: | chromosome 7 |
| Location: | 2669513 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330750 | PHC43 | Putative pherophorin-chlamydomonas homolog; (1 of 1) IPR006311//IPR024616 - Twin-arginine translocation pathway, signal sequence // Pherophorin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGCAGCACGCGTTGTTGGTCGGGCAGC |
| Internal bar code: | GAGTGCGTGCTGGGTGCAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 345 |
| LEAP-Seq percent confirming: | 99.6162 |
| LEAP-Seq n confirming: | 1817 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGATGCTGTCGGTGTAGA |
| Suggested primer 2: | CTCGACATGTGCTACGAGGA |