| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086836 |
| Chromosome: | chromosome 16 |
| Location: | 6838457 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675413 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTAGCAGACCGCCGGCCTACTGTGGTT |
| Internal bar code: | GTCGGCGACATGGTTCGTACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 68.1682 |
| LEAP-Seq n confirming: | 6159 |
| LEAP-Seq n nonconfirming: | 2876 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCGTGTGTATGTGCTAGT |
| Suggested primer 2: | GGGTTCCTGTTGTCATGCTT |