| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086839 |
| Chromosome: | chromosome 12 |
| Location: | 8517682 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547800 | SNE15 | (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; NAD dependent epimerase/dehydratase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTATGTCAACCATGGCTTTTGCTATAGCC |
| Internal bar code: | TGTCGACTGATCTGCACTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 85.3134 |
| LEAP-Seq n confirming: | 4804 |
| LEAP-Seq n nonconfirming: | 827 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACTTGGACTCGTGCTCA |
| Suggested primer 2: | CTTACTTGCCTTCAGCGTCC |