| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086850 |
| Chromosome: | chromosome 3 |
| Location: | 7755901 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203451 | CYA5 | Adenylate cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGAACCAGGAGGTCAGCTTAAAGCCAG |
| Internal bar code: | TTAGAAAAGGTCCCGGATCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 98.9163 |
| LEAP-Seq n confirming: | 5659 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATTGCTTGCTGCCTGTTG |
| Suggested primer 2: | ATGTTCGAGAGACCCATTCG |