| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.086870 |
| Chromosome: | chromosome 10 |
| Location: | 3310707 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443550 | (1 of 1) 2.7.11.11//2.7.11.12 - cAMP-dependent protein kinase / Protein kinase A // cGMP-dependent protein kinase / PKG II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCGGCTGCCCGCTTGCGCTGCTGTACG |
| Internal bar code: | ATCCACGCCGTAGTGCACCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 94.4206 |
| LEAP-Seq n confirming: | 440 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGGGTGTCAAACACAAG |
| Suggested primer 2: | TGCATACCTCATCCTCCTCC |