Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.086870 |
Chromosome: | chromosome_11 |
Location: | 2770715 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g477300 | PPP36 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGCGACTTGACAGCTAATGGCGTGCGCCA |
Internal bar code: | CGGGATGCCGTAGTAACGACCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 96 |
LEAP-Seq percent confirming: | 0.990364 |
LEAP-Seq n confirming: | 74 |
LEAP-Seq n nonconfirming: | 7398 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGTGACCCGGTAAGAGTGC |
Suggested primer 2: | AGCACGCTCAAATCCTGACT |