| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086900 |
| Chromosome: | chromosome 13 |
| Location: | 1129183 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569500 | (1 of 3) PTHR10696 - GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED; Similar to conserved hypothetical protein in bacteria | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGCGCGTGTCCATTGGAAATTACAGCG |
| Internal bar code: | ACGAGGCTCGTTAGCGCGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.7437 |
| LEAP-Seq n confirming: | 3502 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGGTGAGGATGAGGAAA |
| Suggested primer 2: | CTGTCCGTCAACTACAGCGA |