| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086954 |
| Chromosome: | chromosome 3 |
| Location: | 7947838 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202150 | DMC2 | (1 of 6) 2.1.1.37 - DNA (cytosine-5-)-methyltransferase / Type II DNA methylase; Putative DNA methylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGGGGCTGCTGCGGCTCTTGGGCTCGC |
| Internal bar code: | GGTCGTAAGCAAATCAGACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 98.9691 |
| LEAP-Seq n confirming: | 2112 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCACGGATCTTGGTACT |
| Suggested primer 2: | TAAGGGGTCGGCAACAATAC |