| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.086967 |
| Chromosome: | chromosome 13 |
| Location: | 2523756 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580500 | (1 of 2) PTHR12847:SF3 - NECAP-LIKE PROTEIN CG9132 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGCCATGTGGCTAAGTGCGCGCATGA |
| Internal bar code: | CACCGGCTCCAGGTCGACGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 99.6452 |
| LEAP-Seq n confirming: | 5055 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGAGGTCAGATGAGGGGA |
| Suggested primer 2: | ATCATCTCATCGCCACCTTC |