| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.086978 |
| Chromosome: | chromosome 5 |
| Location: | 1732686 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242700 | SUB9 | (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTATTCACGTGCTTCACAGGCGCTGGGA |
| Internal bar code: | ACCTTGGCCTGCAAACTAAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 75.4346 |
| LEAP-Seq n confirming: | 2343 |
| LEAP-Seq n nonconfirming: | 763 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTAGCCAGTTCAACACC |
| Suggested primer 2: | CGCGTGTTAATCACATCACC |