| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086979 |
| Chromosome: | chromosome 1 |
| Location: | 3136751 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019550 | ankryin repeat containing protein; (1 of 2) PTHR24128:SF17 - E3 UBIQUITIN-PROTEIN LIGASE XBAT32 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCATTGTGAGCTGTTGTTGATTGTCC |
| Internal bar code: | CGCGTCTGTCAAGTTCGATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 57.76 |
| LEAP-Seq n confirming: | 361 |
| LEAP-Seq n nonconfirming: | 264 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGCTGAAAGCAAACGTC |
| Suggested primer 2: | CACGGAGCTCCCACTAAAAA |