| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.086997 |
| Chromosome: | chromosome 7 |
| Location: | 5216689 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348800 | OPR32,TAB1 | Translation factor for psaB | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACGCCGTCCAGCGCACATGTACTCCAT |
| Internal bar code: | CATCCGCGCGTATGTCGGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 68 |
| LEAP-Seq percent confirming: | 21.5909 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 207 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTTGAGCGAAAGTCGTG |
| Suggested primer 2: | GGGTGCAGTACCTGGACAGT |