| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.087008 |
| Chromosome: | chromosome 12 |
| Location: | 7592344 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555100 | (1 of 726) IPR011009 - Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCTAGGGTTGTTCCTGGCCTTAGAATG |
| Internal bar code: | CTGGAGGTCGATGGAGTGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 99.5628 |
| LEAP-Seq n confirming: | 1822 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGGTACCTGACCCAGTG |
| Suggested primer 2: | CGAAACCGTAGGTGGATGTT |