| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.087025 |
| Chromosome: | chromosome 1 |
| Location: | 4729448 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032600 | HPAT1,CGL25 | (1 of 14) PF14295 - PAN domain (PAN_4); Hydroxyproline O-arabinosyl transferase 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTGTGCGTGTTCAGCGGCCTTCCCCTT |
| Internal bar code: | ATGTTGGTGTGATCTTCGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 38 |
| LEAP-Seq percent confirming: | 93.9755 |
| LEAP-Seq n confirming: | 12635 |
| LEAP-Seq n nonconfirming: | 810 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACCTCACCAGGTCGTTC |
| Suggested primer 2: | GCATGTTCCACACTGTCACC |