| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.087040 |
| Chromosome: | chromosome 10 |
| Location: | 4508047 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452000 | (1 of 1) PF04752 - ChaC-like protein (ChaC) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGGTGCAGGTGGTGGAGGAAGAGCACC |
| Internal bar code: | TGTGTAACCCTGACGTTACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 394 |
| LEAP-Seq percent confirming: | 7.77027 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 546 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACCCTACGGGACTTTAC |
| Suggested primer 2: | GAGGGGTGCTCTGAGTTCTG |