| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087057 |
| Chromosome: | chromosome 5 |
| Location: | 1625242 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243353 | (1 of 14) PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGGGACAGCAGCAGCTACTGCCGGTCT |
| Internal bar code: | TCATAAAATTCTCCGGGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 99.4652 |
| LEAP-Seq n confirming: | 186 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTCTGAATCTCGGTTGA |
| Suggested primer 2: | CACCCTTGCAATATCCTCGT |