| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.087066 |
| Chromosome: | chromosome 8 |
| Location: | 4110109 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379700 | PHM1 | (1 of 1) 5.4.2.3 - Phosphoacetylglucosamine mutase / N-acetylglucosamine-phosphate mutase; Phosphoacetylglucosamine/phosphohexo mutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGGAGTCACCGGCCCAGTGGCAGGAGC |
| Internal bar code: | GTATGTCTCACGGCCGGCAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 53 |
| LEAP-Seq percent confirming: | 98.0392 |
| LEAP-Seq n confirming: | 250 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGGCTGAAGTAGACCAG |
| Suggested primer 2: | GACAACGGCGTAAAGATGGT |