| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087085 |
| Chromosome: | chromosome 6 |
| Location: | 7159147 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297650 | UBC36 | (1 of 1) K10582 - ubiquitin-conjugating enzyme E2 Q (UBE2Q); E2 ubiquitin conjugating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAATGACGACGTTGGAGGTGCCGTGCCA |
| Internal bar code: | CCTGTGGGGTGTGGTTCTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 371 |
| LEAP-Seq percent confirming: | 99.8328 |
| LEAP-Seq n confirming: | 7763 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCCCATAATGTGTTTTG |
| Suggested primer 2: | CCTGCATGTTGTCAAACACC |