| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087101 |
| Chromosome: | chromosome_7 |
| Location: | 636521 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g316992 | sense | 5'UTR|CDS|intron|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCAGCCCAAACAATCTGTTTACATGCTTGA |
| Internal bar code: | TGGCCTCTACAAAATTCCGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 99.523 |
| LEAP-Seq n confirming: | 1669 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCGGCTCCTTCTACAAT |
| Suggested primer 2: | ATCAAAACACCCCTCAGTGC |