| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087158 |
| Chromosome: | chromosome 10 |
| Location: | 5751097 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461000 | (1 of 1) K14772 - U3 small nucleolar RNA-associated protein 20 (UTP20) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAGGAAGAATGGAGAACACGCAGTGTC |
| Internal bar code: | AACGTCATTGCCTGCATCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 99.2462 |
| LEAP-Seq n confirming: | 395 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGGAGATGATGAAGCGG |
| Suggested primer 2: | GCAGACGCTTACCTTCTTGG |