| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087168 |
| Chromosome: | scaffold 19 |
| Location: | 102070 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750647 | (1 of 1) PF01636//PF07714 - Phosphotransferase enzyme family (APH) // Protein tyrosine kinase (Pkinase_Tyr) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCGCCATAATATGCAGTGCCGTTGCCAT |
| Internal bar code: | TCGAATGCAGTGCCATGGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1100 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGGTTTAGGCCGATTCA |
| Suggested primer 2: | AGGTCGCTTAATGCTCCTGA |