Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.087175 |
Chromosome: | chromosome 13 |
Location: | 2712464 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g582250 | (1 of 1) 3.2.1.96 - Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase / Endo-beta-N-acetylglucosaminidase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAAACACACACGCAGGCCTGGATGCCCC |
Internal bar code: | CCTGTGTAAAGAGGAAAGGGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 305 |
LEAP-Seq percent confirming: | 66.9683 |
LEAP-Seq n confirming: | 148 |
LEAP-Seq n nonconfirming: | 73 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTTGGTCTGGGCTCTGTAG |
Suggested primer 2: | CCAGCCCAAACTAAACCAAA |