| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087210 |
| Chromosome: | chromosome 16 |
| Location: | 4698591 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687117 | (1 of 2) PF14249 - Tocopherol cyclase (Tocopherol_cycl) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCTTGAAGCCTTGAACATTGTATTGAT |
| Internal bar code: | CCAAGCTGCCGTTACCCGTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 95.8042 |
| LEAP-Seq n confirming: | 548 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTTGCAATGGGTTCGATT |
| Suggested primer 2: | CGGCAAGAGACTTGAACTCC |