Insertion junction: LMJ.RY0402.087232_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.087232
Chromosome:chromosome 3
Location:7718403
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g203500 Protein tyrosine kinase; (1 of 107) PF00069//PF07714 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACGAAACCTGGTGCACAGGTCTGCAAA
Internal bar code:TCCACACCGTGATTGTTCGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:440
LEAP-Seq percent confirming:98.0562
LEAP-Seq n confirming:454
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCATGGCTTGTTCATTGT
Suggested primer 2:CTTGTGCCCAATGTGATTTG