| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.087277 |
| Chromosome: | chromosome 12 |
| Location: | 5836468 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533600 | DESI6,DeSI-6 | (1 of 6) PF05903 - PPPDE putative peptidase domain (Peptidase_C97); DeSI-type SUMO protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCGGCGGCCGTCGCAAGGCGCTGCTGC |
| Internal bar code: | GTCGTTAGAATGAGCGCTTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 99.0469 |
| LEAP-Seq n confirming: | 1247 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGACCTCTGCACTGAGC |
| Suggested primer 2: | CCAGACTCTGTCAAGGGCTC |