| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.087288 |
| Chromosome: | chromosome 2 |
| Location: | 6872668 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119650 | GNT3 | (1 of 82) IPR020846 - Major facilitator superfamily domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTGTGTGTCGTGCGTGTCGTGTCGTGTT |
| Internal bar code: | CTGTGTTCATATTCTCTTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 99.3966 |
| LEAP-Seq n confirming: | 9224 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGCACCTGCAGTACAAG |
| Suggested primer 2: | CAGCTACTAATGCTGCTGCG |