| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087304 |
| Chromosome: | chromosome 12 |
| Location: | 3380869 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497100 | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGCTGAGGTCGCGCGAGGAGTGATGTC |
| Internal bar code: | CGGACCCGCATGTATGAAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 97.5142 |
| LEAP-Seq n confirming: | 3256 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTCCAGGCGTTTGAACT |
| Suggested primer 2: | TAGGCGTGTGGAATGACAAA |