| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.087306 |
| Chromosome: | chromosome 9 |
| Location: | 5114508 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398808 | (1 of 2) PF01465 - GRIP domain (GRIP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTACTCGGCGTCGATGATGTTGGCGCC |
| Internal bar code: | CGAGTCGCTCGCATGTTGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 99.7569 |
| LEAP-Seq n confirming: | 4103 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTACAGGATGCGCTGAAG |
| Suggested primer 2: | TCACAACCACCACGACATCT |