Insertion junction: LMJ.RY0402.087309_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.087309
Chromosome:chromosome 3
Location:1973484
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g155750 PHC65 Putative pherophorin-chlamydomonas homolog; (1 of 1) IPR001304//IPR016186//IPR016187//IPR018081//IPR024616 - C-type lectin // C-type lectin-like // C-type lectin fold // Anaphylatoxin, complement system // Pherophorin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACCGCCTCGCCTGCCCGCATACACAGCG
Internal bar code:CCCTAAGAGGAATGACTGGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:123
LEAP-Seq percent confirming:19.5554
LEAP-Seq n confirming:475
LEAP-Seq n nonconfirming:1954
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCCTTAAACCCGTACGAC
Suggested primer 2:GTACTGTCCCCACCACCTGT