Insertion junction: LMJ.RY0402.087323_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.087323
Chromosome:chromosome 1
Location:5846784
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g041752 (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACAGCACACACCAACGAACCCCACTTAC
Internal bar code:TGGTCGATGTTACTGCTAACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2643
LEAP-Seq percent confirming:98.3222
LEAP-Seq n confirming:293
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGCACACAACAAAACGTGTC
Suggested primer 2:CAGCACTCAGTTCCTGGTCA