| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.087336 |
| Chromosome: | chromosome_17 |
| Location: | 2404921 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g715300 | PKD2 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCACCATCGAGTCGGTCGCGGCGCCCAGCG |
| Internal bar code: | GTCCGGACCGGGATAACATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 82.6578 |
| LEAP-Seq n confirming: | 1244 |
| LEAP-Seq n nonconfirming: | 261 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACATGGTTCGTAGCGTTT |
| Suggested primer 2: | CGAATCTGCTATACGACGCA |