Insertion junction: LMJ.RY0402.087346_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.087346
Chromosome:chromosome 9
Location:3927578
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g392319 VSP6 (1 of 8) PTHR11339//PTHR11339:SF290 - EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED // PROTEIN T26A8.1; Hydroxyproline-rich cell wall glycoprotein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGAGTGCCCATGCGAGTCTGCTTCTCTCC
Internal bar code:CGGCTACATATTATAGGATATT

Confirmation - LEAP-Seq

LEAP-Seq distance:365
LEAP-Seq percent confirming:99.6969
LEAP-Seq n confirming:3289
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AAAACGTGTCCGAGCATACC
Suggested primer 2:TCGTGGGGTAGCCTGATTAC