| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087369 |
| Chromosome: | chromosome 3 |
| Location: | 5223795 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182800 | AGT2 | Alanine-glyoxylate transaminase; (1 of 1) K00827 - alanine-glyoxylate transaminase / (R)-3-amino-2-methylpropionate-pyruvate transaminase (AGXT2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGGGCGGCCTGCACGGCAACGTGTTCC |
| Internal bar code: | TTTGACTCATGTTGCAACGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.7323 |
| LEAP-Seq n confirming: | 6705 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCAAACGCGGAGATTTAG |
| Suggested primer 2: | CCAACAGAGGAGGGTTTCAG |