Insertion junction: LMJ.RY0402.087372_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.087372
Chromosome:chromosome 9
Location:7925572
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g417250 ELG27 Exostosin-like glycosyltransferase 27; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAATAATTGAGTGCTTGTTGCCCATCAT
Internal bar code:CGCAAATTGGGTCCAATCCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:131
LEAP-Seq percent confirming:55.5556
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACTAAACGCACAACGATCCC
Suggested primer 2:TCAGGTATGCACCTGCTGAG