| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087374 |
| Chromosome: | chromosome 1 |
| Location: | 325463 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001950 | Predicted protein with 3-phosphoshikimate1-carboxyvinyltransferase core domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTTATATCATGCCGCACTAGAGGAGAC |
| Internal bar code: | GGTGCTAACCCAACGATGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 990 |
| LEAP-Seq percent confirming: | 99.6744 |
| LEAP-Seq n confirming: | 3673 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTTGCCTCACAAGTTGC |
| Suggested primer 2: | GGAGATGCCTCCATGTCTGT |