| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.087382 |
| Chromosome: | chromosome 10 |
| Location: | 4720850 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453650 | (1 of 1) PTHR32086//PTHR32086:SF0 - FAMILY NOT NAMED // FANCONI ANEMIA GROUP D2 PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCCACCTGCCTACGGACACGCTGCCTT |
| Internal bar code: | TCGAATCAAGCTACCGTGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTGCCAACAGTCTGCAC |
| Suggested primer 2: | CCCTACACCCACACCCATAC |