| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.087383 |
| Chromosome: | chromosome 12 |
| Location: | 2421828 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507051 | (1 of 2) K01193 - beta-fructofuranosidase (E3.2.1.26, sacA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCAGGCGTTGTGGACGGCGAGCAGTGT |
| Internal bar code: | GGGGGGTCGCTTGGTGCGAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 572 |
| LEAP-Seq percent confirming: | 99.1834 |
| LEAP-Seq n confirming: | 3644 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGTTTGGAAAAGCGGTA |
| Suggested primer 2: | GGCTACCCCACCTCCTACAT |