| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.087399 |
| Chromosome: | chromosome 6 |
| Location: | 1246664 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257900 | (1 of 1) K10088 - protein OS-9 (OS9) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGTCGATCGAAAGCAGCAGGCTGAGAC |
| Internal bar code: | GCTTCAACTATTGATCACATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1237 |
| LEAP-Seq percent confirming: | 95.9821 |
| LEAP-Seq n confirming: | 215 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACGACAGAGATGTCGAA |
| Suggested primer 2: | TATCATTTCCAGAGCCGACC |