| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.087404 |
| Chromosome: | chromosome 17 |
| Location: | 4155167 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729850 | PF6-IP4,C1a-18,FAP227 | Flagellar central pair-Associated Protein 227; (1 of 5) 2.7.1.68 - 1-phosphatidylinositol-4-phosphate 5-kinase / Type I PIP kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAATAGTCCTACTCCATCCAAGCTCTGC |
| Internal bar code: | GCGGAGACGAGGGAGCGCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 90 |
| LEAP-Seq percent confirming: | 89.8399 |
| LEAP-Seq n confirming: | 1291 |
| LEAP-Seq n nonconfirming: | 146 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAATGTTTTTGGCAAGGAA |
| Suggested primer 2: | TACGTACGTGGCGTATGGAA |