Insertion junction: LMJ.RY0402.087428_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.087428
Chromosome:chromosome 6
Location:2350980
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g267450 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCGTGCTTCGGCGGTCTGGGAATGAGC
Internal bar code:ACGTGGTACGTCACATATCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:635
LEAP-Seq percent confirming:99.5131
LEAP-Seq n confirming:2248
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAAGGACAAGGTGGTCAG
Suggested primer 2:CTGGAAGTGAACTGGCAACA