| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087453 |
| Chromosome: | chromosome 12 |
| Location: | 464202 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493500 | SOX1 | (1 of 1) PTHR10961 - PEROXISOMAL SARCOSINE OXIDASE; Sarcosine oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGGCTCCATTCACCTCAGGTAAGATTGT |
| Internal bar code: | CGTCTATCTCCCATCGCATCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 86.385 |
| LEAP-Seq n confirming: | 552 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAACGAGGTGTGGTGTGGT |
| Suggested primer 2: | GGCAGTGGAGGTTGGAAGTA |