| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087465 |
| Chromosome: | chromosome_1 |
| Location: | 6935288 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g049950 | VLE1,SPO | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTAGAACGAGCCCCCAAAGTCCACAAATGA |
| Internal bar code: | GCCGAGGCCTCCAATTTCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1087 |
| LEAP-Seq percent confirming: | 99.7101 |
| LEAP-Seq n confirming: | 4472 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACTTCGCTGCAATGGTG |
| Suggested primer 2: | AAGAGCGAGTCAGTCGGGTA |