Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.087465 |
Chromosome: | chromosome_12 |
Location: | 3547748 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g513400 | EBG1 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCCATGCCTGGCGTCCGCTGCGTTACCGGT |
Internal bar code: | TCTGTTTGGAAACTCTCGGGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 666 |
LEAP-Seq percent confirming: | 98.98 |
LEAP-Seq n confirming: | 2329 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCACCGTCAGTAACAACACG |
Suggested primer 2: | GTCACACGCTGGCTGAAGTA |