Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.087481 |
Chromosome: | chromosome_12 |
Location: | 2573404 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g505350 | ACA2 | P-type ATPase/cation transporter, plasma membrane | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGATCGAGTAACTTGTTATCTATGAAGGTA |
Internal bar code: | CATCTAGGGGTGAGTGTGTGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 837 |
LEAP-Seq percent confirming: | 99.7781 |
LEAP-Seq n confirming: | 4497 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTATGCGGTACCATGCACT |
Suggested primer 2: | TACGGACACCACAAGGATGA |