Insertion junction: LMJ.RY0402.087548_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.087548
Chromosome:chromosome 17
Location:2806286
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g718900 FAP88 Flagellar Associated Protein 88; (1 of 1) PTHR23237//PTHR23237:SF6 - NUCLEOLAR PROTEIN FAMILY A MEMBER 1 SNORNP PROTEIN GAR1 // H/ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACGTGCCCCGACGGCCCGCGCACCTTGC
Internal bar code:AGAGTCAGACTGCTGCGCGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:490
LEAP-Seq percent confirming:99.8238
LEAP-Seq n confirming:5099
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGTAAGAAGGTGGACAGC
Suggested primer 2:ATGTTATAAACCCCTCCCGC