| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087552 |
| Chromosome: | chromosome 3 |
| Location: | 6460454 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194800 | (1 of 1) K14399 - polyribonucleotide 5'-hydroxyl-kinase (CLP1, HERB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGACCCGCACCACGTGCCCATCCCAGCC |
| Internal bar code: | GGGAGCAGTGTTTCCGAGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 88.75 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATCTGAAGAAGGCGTGA |
| Suggested primer 2: | CTTCTACGGACACTCCTCGC |