| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.087579 |
| Chromosome: | chromosome 7 |
| Location: | 5792576 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353150 | (1 of 4) PTHR10229//PTHR10229:SF0 - GTP-BINDING PROTEIN HFLX // GTP-BINDING PROTEIN 6-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGCCAGTCGCCCCCATCGCCTCACCGC |
| Internal bar code: | TACTCCTTCGCTCTGAGGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 98.9537 |
| LEAP-Seq n confirming: | 662 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCGAGTGGTGCAGAGTA |
| Suggested primer 2: | CCTACCTCTCGCAGTTCCAG |