| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087597 |
| Chromosome: | chromosome_1 |
| Location: | 5532884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g039200 | IFT22,FAP9 | Intraflagellar transport protein 22 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGAGACCTACTGTGCTTGGCAACCAACCC |
| Internal bar code: | AGAGACTGCGATGCTTTTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 98.5448 |
| LEAP-Seq n confirming: | 2167 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGAGGACTGGGAAGACG |
| Suggested primer 2: | CGTGAGACGACTTGCGATTA |