| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.087622 |
| Chromosome: | chromosome 12 |
| Location: | 801170 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495850 | HBD1 | (1 of 1) PTHR24322//PTHR24322:SF513 - FAMILY NOT NAMED // PROTEIN DHS-2, ISOFORM A; 3-hydroxybutyrate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGACGACGGCTATCTGCTGCTCTGCCCC |
| Internal bar code: | TCCGCTCTTCACGGATCTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 98.2759 |
| LEAP-Seq n confirming: | 171 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTGGAGAGCACATCTGG |
| Suggested primer 2: | CTGCCCTGTATGGAATGCTT |